Normal human peripheral blood cells were unstimulated (left) or stimulated 5 hours with LPS in the presence of Protein Transport Inhibitor Cocktail (Product # 00-4980-03) (right). Cells were intracellularly stained with Anti-Human CD14 PE (Product # 12-0149-42) and Anti-Human CCL7 eFluor® 660 using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Cells in the monocyte gate were used for analysis.